Dataset: Moosilauke Data: Lodge 2001-2003

Methods

The methods followed in the creation of the dataset, including description of field, laboratory and processing steps, and quality control procedures

Current Methods

• Experimental design
  Started: 0001-01-01
  

  A split-split plot experimental design was used on the plots, split by species (balsam vs fir) first and then samples were split by crown position (sun vs shade). After sample processing, three needles from each sample were used for anatomical measurements.

  Instrumentation: R:FR meter (Skye instruments);

  Software: Leica Q500MC and QWin V1.00 (Leica Cambridge Ltd.)

• Photosynthetic rates
  
  

  Photosynthesis measurements were made on detached shoots with their ends immersed in vials of water. Projected needle area and dry mass were measured and then used to convert the measured photosynthetic rates to projected needle area. Chl NDI and PRI indices were used to obtain physiological insights from the chlorophyll fluorescence and spectral reflectance of dark-adapted foliage.

  Instrumentation: OS-500 Modulated Fluorometer UniSpec Spectral Analysis System

• Foliar C-13 analysis
  
  

  Using a pool of three samples from each site x crown position x species combination, an analysis of foliar C-13 was conducted by a commercial laboratory.

  Instrumentation: Europa Scientific ANCA-GSL elemental analyzer

  Quality Control Measures: Two replicated of every fifth sample were analyzed sequentially. All samples were run against a flour standard and results are expressed in relation to the PDB standard.

Dataset Fields

Detailed documentation of the fields comprising the dataset, including the type of measurement, units where applicable, and any controlled vocabularies or code lists present in the data

Sampling Equipment

Equipment and software used to collect data, including how that equipment was used.

Past Sampling Equipment (No longer in use)

• Data logger, humidity sensor
  Started: 2000-10-01
  Ended: 2002-09-30

  Hobo H8 Pro data logger with integral thermistor for air temperature and capacitive sensor for relative humidity.

• Air and soil temperature
  Started: 2001-09-01
  Ended: 2002-09-30

  Copper-contantan thermocouples (in conjunction with CR10XTCR (CSI) reference thermocouple) for air temperature and soil temperature (15 cm depth)

  Frequency: 10 s sampling interval, 15 minute means output to storage

• Relative Humidity
  Started: 2001-09-01
  Ended: 2002-09-30

  CS 500 at 748 m, HMP35C at 1425 m; both CSI

  Frequency: 10 s sampling interval, 15 minute means output to storage

• Solar Radiation
  Started: 2001-09-01
  Ended: 2002-09-30

  Quantum sensor (190SZ, Li-Cor Inc., Lincoln, NE) and pyranometer (Model 50, Eppley Laboratory, Newport, RI; 748 m elevation only)

  Frequency: 10 s sampling interval, 15 minute means output to storage

• Wind Speed and Direction
  Started: 2001-09-01
  Ended: 2002-09-30

  03001 Wind Sentry, R.M. Young Co., Traverse City, MI

  Frequency: 10 s sampling interval, 15 minute means output to storage

Site Characteristics

The spatial extent of the dataset site coverage, and descriptions of the spatial extent and context for the data collection

• Site Description

  Sampling sites were located on three mountains (Whiteface Mt., Mt. Mansfield and Mt. Moosilauke), along two transects on each mountain (eastern and southern), at three elevations on each transect (low, mid and high elevation), for two species (red spruce and balsam fir) and two crown positions (sun and shade).