• Address:
  705 Spear Street
  South Burlington, Vermont 05403
  United States of America
  
  Phone: (802) 391-4135
  Email: femc@uvm.edu
  Website: www.uvm.edu/femc

Determine the response of winter algal communities to changes in environmental conditions: flow, light and nutrient availability.

• Algal samples
  • Started: 1994-08-01
    Ended: 1995-03-31
    
  • Method Description: Algal samples were collected between August 1994 and January 1995, and cell densities of 3 taxa were counted: Chamaesiphon sp., Lyngbya subtilits, and Synechocystis sp. as a baseline for diversity. Algal samples were also collected on 3 occasions during February and March 1995, using low light, high light and slow flow, fast flow treatments.

• Measuring effects of light and flow
  • Started: 1994-01-01
    Ended: 1994-04-05
    
  • Method Description: The effect of a 300-450% difference in light and doubling of flow on periphyton was tested by collecting 100 or more stream rocks, removing periphyton and processing it for chlorophyll a and ash free dry mass.

• Treatment effects on colonization
  • Started: 1994-01-01
    Ended: 1994-04-05
    
  • Method Description: 100 uncolonized clay tiles were added to each treatment and collected at the end of the experiment to determine the treatment effects on colonization.

• Nutrient availability
  • Started: 1994-02-04
    Ended: 1994-03-17
    
  • Method Description: Nutrient availability was determined using stream water samples analyzed for nitrate, ammonia, soluble reactive phosphorus, and total phosphorus.

• Determination of periphyon limiting factor
  • Started: 1995-01-05
    Ended: 2004-08-02
    
  • Method Description: Clay saucers filled with agar containing N, P, N & P or no nutrients were submerged in the stream for 2 months, then sampled for chlorophyll a and ash free dry mass to determine whether periphyon were nitrogen or phosphorus limited.

• Unnamed Method
• Started: 1995-01-05
  Ended: 2004-08-02
  
• Method Description: Ended low light treatments