• Address:
  705 Spear Street
  South Burlington, Vermont 05403
  United States of America
  
  Phone: (802) 391-4135
  Email: femc@uvm.edu
  Website: www.uvm.edu/femc

To better understand the relationship between plants and their abiotic environment, by using the response of three different tree species growing at different elevations, to light.

• Label: Observation

• Description: Observation ID Number

• Storage Type: int

• Measurment Type: nominal

• Label: Species

• Description: Species (spruce or fir)

• Storage Type: text

• Measurment Type: nominal

• Label: Elevation

• Description: Elevation level (low, mid and high)

• Storage Type: text

• Measurment Type: nominal

• Label: Crown Position

• Description: Crown position (sun or shade)

• Storage Type: text

• Measurment Type: nominal

• Label: Tree ID

• Description: Tree ID

• Storage Type: text

• Measurment Type: nominal

• Label: Transect

• Description: Transect ID

• Storage Type: int

• Measurment Type: nominal

• Label: Mountain

• Description: Name of mountain where study site is located

• Storage Type: text

• Measurment Type: nominal

• Label: Sample Code

• Description: Code unique to sample

• Storage Type: text

• Measurment Type: nominal

• Label: Needle Mass

• Description: Mass of needle

• Storage Type: decimal

• Measurment Type: ratio
• Standard Unit: milligram
• Precision: 0.0001
• Unit: milligram
• Number Type: real

• Label: Needle Area

• Description: Area of needle

• Storage Type: decimal

• Measurment Type: ratio
• Standard Unit: squareCentimeters
• Precision: 0.001
• Unit: squareCentimeters
• Number Type: real

• Label: Needle Length

• Description: Length of needle

• Storage Type: decimal

• Measurment Type: ratio
• Standard Unit: centimeter
• Precision: 0.01
• Unit: centimeter
• Number Type: real

• Label: Chlorophyll Fluorescence

• Description: Chlorophyll fluorescence

• Storage Type: decimal

• Measurment Type: ratio
• Standard Unit: meter
• Precision: 0.001
• Unit: meter
• Number Type: real

• Label: Photochemical Reflectance Index

• Description: Photochemical reflectance index; a measure of photosynthetic radiation use efficiency

• Storage Type: decimal

• Measurment Type: ordinal

• Label: Red Edge Position

• Description: Chlorophyll measure

• Storage Type: decimal

• Measurment Type: ordinal

• Label: ChI NDI

• Description: Chlorophyll-based difference index

• Storage Type: decimal

• Measurment Type: ordinal

• Label: Asat

• Description: unknown

• Storage Type: decimal

• Measurment Type: ratio
• Standard Unit: unknown
• Precision: 0.01
• Number Type: real

• Label: Comments

• Description: Comments

• Storage Type: text

• Measurment Type: nominal

• Experimental design
  • Started: 0001-01-01
    
  • Method Description: A split-split plot experimental design was used on the plots, split by species (balsam vs fir) first and then samples were split by crown position (sun vs shade). After sample processing, three needles from each sample were used for anatomical measurements.
  Instrumentation: R:FR meter (Skye instruments); Software: Leica Q500MC and QWin V1.00 (Leica Cambridge Ltd.)

• Photosynthetic rates
  • Method Description: Photosynthesis measurements were made on detached shoots with their ends immersed in vials of water. Projected needle area and dry mass were measured and then used to convert the measured photosynthetic rates to projected needle area. Chl NDI and PRI indices were used to obtain physiological insights from the chlorophyll fluorescence and spectral reflectance of dark-adapted foliage.
  Instrumentation: OS-500 Modulated Fluorometer UniSpec Spectral Analysis System

• Foliar C-13 analysis
  • Method Description: Using a pool of three samples from each site x crown position x species combination, an analysis of foliar C-13 was conducted by a commercial laboratory.
  Instrumentation: Europa Scientific ANCA-GSL elemental analyzerQuality Control Measures: Two replicated of every fifth sample were analyzed sequentially. All samples were run against a flour standard and results are expressed in relation to the PDB standard.