Forest Ecosystem Monitoring Cooperative

Biomolecular Indicators of Acid Stress: Indicators of Acid Stress in Northeastern Forest Soil Methane Oxidizing Communities

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Metadata Provider

  • Forest Ecosystem Monitoring Cooperative

    • Address:
      705 Spear Street
      South Burlington, Vermont 05403
      United States of America

      Phone: (802) 391-4135
      Email: femc@uvm.edu
      Website: www.uvm.edu/femc

Abstract

    Investigate the effects of air quality, especially acid deposition, on terrestrial and aquatic ecosystems, and particularly on microbial communities.Study of the application of molecular biology techniques to investigate diversity, community structure, and function of methane oxidizing bacteria and nitrifying bacteria in forest soils affected by atmospheric acid deposition.

People

  • Mark Bremer: Principal Investigator

Keywords

  • acid deposition, biomolecular, soil bacteria, soil chemistry, water chemistry,

Organizations

  • Syracuse University : lead

Geographic Coverage

  • Coordinates

    Data Table

    • Title: Indicators of Acid Stress in Northeastern Forest Soil Methane Oxidizing Communities
    • Start Date: 2001-01-01
    • End Date: 2002-01-01
    • Description: Investigate the effects of air quality, especially acid deposition, on microbial ecosystems.

    • Purpose: Specific objectives include assessing the diversity and community structure of soil bacteria (nitrifiers and methanotrophs) in different acid depostion environments; develop a biomolecular method for determining ecosystem acid stress; and validate the PnET-BGC forest-soil-water model on field, GIS and remote sensing data then regionally apply the model.

    • Condensed Title:

    • Object Name: VMC.154.1154

    • Data Type: mySQL
    • Citation: Mark Bremer. 2003. Indicators of Acid Stress in Northeastern Forest Soil Methane Oxidizing Communities. FEMC. Thesis, https://www.uvm.edu/femc/data/archive/project/biomolecular-indicators-acid-stress/dataset/Indicators-of-Acid-Stress-in-Northeastern-Forest-Soil-Methane-Oxidizing-Communities

    • Online Distribution: https://vmc.w3.uvm.edu/vmcdevel/data/archive/project/biomolecular-indicators-acid-stress/dataset/Indicators-of-Acid-Stress-in-Northeastern-Forest-Soil-Methane-Oxidizing-Communities

    Attribute List

    Methods

    • Past Methods (no longer in use)
      • Site establishment and sample collection
        • Started: 2002-06-10
          Ended: 2002-06-11
        • Method Description: Establish 1 soil pit for every 100m elevation change. Mark and GPS each site; dig to mineral layer; collect 100 g of each horizon (O-B) and pool w/equivalent horizons. Collect 2 250 mL of stream H2O every 200m along a stream. Extract DNA from soil samples, use diagnostic genes to identify nitrifiers and methanotrophs, DNA fingerprint portions of those genes.

    Sampling Equipment

    • Soil
      • Started: 2002-06-10
        Ended: 2002-12-31
      • Sampling Description: Soil sampling
      • Frequency: 75 samples collected 50 g each at 3 soil horizons; collected at 3 elevations/site for each site

    • Water Samples
      • Started: 2002-06-10
        Ended: 2002-12-31
      • Sampling Description: Samples for water chemistry
      • Frequency: 80 samples collected; 500 mL at 5 elevations per site or about every 200 m

    Site Characteristics

    • Site Description: Hubbard Brook Experimental Forest, NH

    • Minimum Altitude: 550 meters

    • Maximum Altitude: 790 meters

    • Site Description: Lye Brook Wilderness Area, VT

    • Minimum Altitude: 580 meters

    • Maximum Altitude: 760 meters

    • Site Description: Willowemoc Creek, NY

    • Minimum Altitude: 520 meters

    • Maximum Altitude: 700 meters

    • Site Description: Bear Brook Watershed, ME

    • Minimum Altitude: 265 meters

    • Maximum Altitude: 475 meters